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This conclusion is supported by multiple lines of experimental data: PA treatment triggers significant ROS generation, depletes intracellular GSH, increases lipid peroxidation (indicated by elevated MDA levels), and causes accumulation of intracellular labile ferrous iron (Fe2+)

(2015) Impact of alginate-producing Pseudomonas aeruginosa on alveolar macrophage apoptotic cell clearance

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Immunofluorescence and western blotting, together with well-characterized antibodies such as GT335 (glutamylation), PolyE, B3, and 1D5 (poly- or long-chain glutamylation), enable visualization and semi-quantitative analysis of different modification states in cells and tissues [3, 36,37,38,39]

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