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Unfortunately for some, this enviable body is hard to come by using traditional weight loss methods and dieting without the inevitable need for medical intervention

doi: 10.1017/s1092852900024901

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Subsequent quantitative reverse transcriptase PCRs were performed using the StepOne Plus RT-PCR system (Applied Biosystems, Waltham, MA, USA) with SYBR Premix Ex Taq II (Takara Bio, Kusatsu, Japan) and primers for amplifying human ALP , VEGFA , and the reference gene GAPDH (see Table 1)

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While 16% of patients with a history of variceal bleeding had recurrent bleeds, no one without such history developed new bleeding