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To determine the expected elution volume of PC-PLC, we performed a calibration of the HiLoad 16/600 Superdex 75 pg column using same buffer, and protein standards (IgG 150 kDa, transferrin 75 kDa, ovalbumin 43 kDa, scFvs 25 kDa, aprotinin 6.5 kDa)

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