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The membranes were blocked in 5% bovine serum albumin (BSA) in TBS-T at room temperature for 1 hour and incubated with the primary antibody directed against 4HNE (ab46545, Abcam

25,26 Finally, Under the action of the enzyme cystathionine--synthase (CBS), serine is coupled into homocysteine to form cystathionine, which can then be cleaved by cystathionine c-lyase (CTH) to release cysteine

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Maintain institutional credibility: Regulatory agencies must resist political pressure (Jasanoff, 2013[57])