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In addition, 47 genes were downregulated in the recombinant strain at all three time points, including the hsp1 heat shock protein-coding gene, the trxA1 thioredoxin-coding gene, and the dinP, mutY, umuC, and uvrB DNA damage repair-related protein-coding genes, potentially indicating that the recombinant strain was less susceptible to stress and had less DNA damage than the control strain in acid stress conditions

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For active B12 deficiency, most clinicians recommend weekly or biweekly shots for the first 4 to 6 weeks, then a maintenance shot every 1 to 3 months

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