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Adenine nucleotides (Sigma-Aldrich) were added to the pipette solution shortly before the experiments and the pH was additionally adjusted

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Heat shock also induced transcriptional reporters of several autophagy genes, including the phosphoinositide-binding protein atg-18 , the SQSTM1/p62 orthologue sqst-1 and atg-16.2 , which is involved in phagophore formation (Supplementary Fig

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