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Description
Mechanical loading mediates human nucleus pulposus cell viability and extracellular matrix metabolism by activating of NF-B

Taurine is involved in mitochondrial homeostasis via its ability to act as a mitochondrial matrix pH buffer, by regulating mitochondrial potential, by modulating Ca 2+ -induced mitochondrial swelling, and by regulating the activity of mitochondrial dehydrogenases and ATP concentration

The primary function of mitochondria is the process of oxidative phosphorylation, which controls ROS production and ATP synthesis to provide cells with energy

Gupta, -L-glutamyltaurine, Amino Acids 28(4) (2005) 343-356

Pernicious anemia is one of the main root causes of B12 deficiency, which affects 5% of the general population and 10%20% of people aged 65 years + (Lacombe V, 2024)

If it is suspected that the expressed protein is toxic to the cells (usually they will stop growing after induction with IPTG), try inducing at a later time point for a shorter amount of time
