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10.1038/hr.2012.163 46 TanejaS

Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min

Furthermore, the aggregation of DJ-1 protein is implicated in PD pathogenesis and increased insolubility of DJ-1 protein has been identified in sporadic PD patient brains [142]

It is indispensable for cell proliferation and tissue growth during fetal development, and folate deficiency during the periconceptional period is linked to a higher risk of neural tube defects (NTDs) in the developing fetus [21]

Orthopedic surgeons understand the risks associated with joint replacement surgery, and are aware that patients with obesity have greater complications after surgery

10.1016/j.jaci.2021.09.024