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Animal preparation and housing Sixty 10-week-old male Sprague-Dawley rats, weighing ~250300 g, were procured from Beijing Vital River Laboratory Animal Technology Co., Ltd

Erythrocyte catalase activity Erythrocyte catalase (e-CAT) activity was determined with a spectrophotometric assay at 240 nm (25 C)

Compromised detoxification: The liver may struggle to remove toxins efficiently

If multiple glutathione deficiency symptoms are showing up at once, like fatigue, poor sleep, and chronic pain, it may be time to look at whats depleting your antioxidant defenses

Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4

Estimation of antioxidant enzyme activities A leaf of fresh plant material weighing 0.2 g was taken and homogenized in phosphate buffer (5 mL) and liquid nitrogen having a pH of neutral