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The medium used to maintain the pellet was full chondrogenic medium (CM) consisted of DMEM (Gibco), 2 anti-anti (Gibco), 10 g/ml ITS (Thermo Fisher Scientific), 0.1 M Dexamethasone (Sigma-Aldrich, St

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Wiiliams Check the amount of vitamin b12 in body before and after the therapy

All samples were run in technical triplicate and each developmental exposure group (i.e., adult, 72 hpf, 7 dpf exposure) were run on independent plates containing all treatments, tissue types, three genes of interest, and the hkg

For that reason, we dont use rubber closures