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Cells were initially pre-cultured in 5 ml of YPDA or SCDropout medium (adjusted to meet auxotrophic requirements for plasmid maintenance) in a cell culture tube rotating overnight at 30 C

Therefore, all the scientific information on its effects is only available from animal and in vitro studies

The medical care team contacted the patient within 24 h

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doi: 10.1017/S0007114522002343 19

Thus, it is important to ensure efficient levels of B12 are maintained within the body as women get older