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Prognosis Most localized injection site reactionslike redness, swelling, or itchingclear up on their own within a few days without lasting effects

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10.1016/S0896-6273(03)00769-4 94 GoodallG.GheusiG

The cells were washed with PBS twice before staining with X-gal solution [1 mg/mL 5-bromo-4-chloro-3inolyl--D-galactoside (Boehringer Manneheim #745-740) in N,N-dimethylformamide, 5 mM potassium ferricyanide crystalline (#P-8131), 5 mM potassium ferricyanide trihydrate (#P-3289), 2 mM magnesium chloride, and 1x PBS, pH 6.0