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The buffer solution in Example 15 contained 0.135 M HCO 2 H and 0.215 M HCO 2 Na and had a pH of 3.95

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Acknowledgements We thank Anna Altin, Tamara Grossrieder, Arne Hansen, Samanda Krasniqi, Marina Reist, Melanie Schulz and Jelena Velimirovic, for their help in data acquisition, and Samuel Gerster for technical support

Standard protocols use insulin syringes with 29 or 30 gauge needles, minimizing discomfort and allowing self-administration
