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The resulting suspension was then centrifuged (20,000x g, 4C) before the supernatant was stored at 4C until further processing
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After addition of the antibodies, cells were incubated at 4C for 20 min in the dark
However, in some individuals, this tolerance to self-tissue is lost
Am J Pathol 2011
Together, our study establishes a ChaC1-based pharmacological discovery platform that identifies proteasome inhibitor and auranofin combination as a mechanistically rational anti-HCC strategy, providing both methodological innovation in drug repurposing screening and immediate translational potential for HCC treatment
