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Autophagy is increased in mice after traumatic brain injury and is detectable in human brain after trauma and critical illness

inactive mutant forms of CDO (e.g., His86Ala, leading to loss of the His that serves as a metal ligand in the active site) did not form any cross-link, and mutant forms of CDO with low activity (e.g., Arg60Ala, which has a markedly decreased affinity for Cys, probably due to the loss of the hydrogen bonding partner for the carboxylate of the substrate) formed the cross-link more slowly

That translates to about 35 pounds for someone starting at 220 pounds

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