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The Hepatocyte Differentiation Basal Medium was composed of DMEM/F12, 10% KnockOut Serum Replacement (Thermo Fisher Scientific, Inc.), 1X NEAA, 1X PEST and 1X GlutaMAX Supplement (Thermo Fisher Scientific, Inc.)

DO NOT USE IF SAFETY SEAL IS DAMAGED OR MISSING
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An immediate and then sustained effect on pressure in lower esophageal sphincter, and a postponed effect on pyloric sphincter after either 0.5 mg/kg or 1.0 mg/kg intraperitoneal challenge (white bars), pyloric sphincter seems to be less affected
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See Chapter 20, Paragraph 20.4.5
