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This hypermethylation is associated with a significant decrease in the expression of these genes, reaching multiple orders of magnitude lower levels compared to primary human hepatocyte cultures

doi:10.1111/jhn.12648 Blinnikova K, Cohen CW, McKeag ID

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Stained cells were detached from the plate surface and suspended in PBS for flow cytometric analysis using the FL1-H and FL2-H channels of a BD FACSCalibur Flow Cytometer (BD Biosciences) with BD CellQuest Pro software

4.2.3 Muscle and adipose tissue fibrosis Tissue fibrosis, characterized by the excessive deposition of extracellular matrix components, is a common pathological outcome in numerous chronic diseases

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