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Summary: Bacteriostatic Water for Injection is essentially a reusable sterile water diluent due to its benzyl alcohol preservative

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Cells were seeded in a 24-well plate at a density of 2x106 cells/ml in a complete medium consisting of RPMI 1640 (Gibco, Grand Island, NY), 10% fetal calf serum (Atlanta Biologics, Atlanta, GA), 100 U/ml of penicillin G sodium (Gibco), 100 g/ml of streptomycin sulphate (Gibco), 0.25 g/ml of amphotericin B (Gibco), and 2 mM L-glutamine (Gibco).The following in vitro treatments were conducted in triplicate wells for each animal: PBS (negative control), pokeweed mitogen (10 g/ml

For a 2mg vial, adding 0.8mL of bacteriostatic water creates a 2.5mg/mL concentration

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Some studies have suggested that it may support fat metabolism, modest fat loss, and healthy body composition while maintaining a relatively favorable safety profile