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This work was supported by the National Natural Science Foundation of China (82472525 and 82272567), Jiangsu Medical Research Project (ZD2022014), Program of Suzhou Health Commission (GSWS2022002), National and Local Engineering Laboratory of New Functional Polymer Materials (SDGC2205), Foundation of National Center for Translational Medicine (Shanghai) SHU Branch (SUITM202403), the Project of MOE Key Laboratory of Geriatric Diseases and Immunology (KJS2502), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX25_1798), Suzhou Municipal Medical and Health Science and Technology Innovation Project (SKY2021023), and Ke Jiao Xing Wei Plan of Wujiang District (WWK202005)

These included water from Honeywell Burdick & Jackson (Muskegon, MI, USA), methyl tert-butyl ether (MTBE) from VWR (Radnor, PA, USA), formic acid from ThermoFisher Scientific (Waltham, MA, USA), acetonitrile and methanol from Fisher Scientific (Hampton, NH, USA), 2-Propanol from Millipore Sigma (Burlington, MA, USA), and InfinityLab Deactivator Additive from Agilent Technologies (Santa Clara, CA, USA)

It directly binds to Nrf2 and promotes GPX4 transcription, which in turn inhibits ferroptosis after IRI and ameliorates brain damage (Choi et al., 2023)

Increased needs

Studies compared with healthy controls by rRNA community profiling, are included and described in Table 1

Apart from its involvement in enzymatic functions, copper also plays a vital role in various physiological processes