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Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)

Shimojo M, Courchet J, Pieraut S, Torabi-Rander N, Sando, R., 3 rd , Polleux F, Maximov A (2015) SNAREs controlling vesicular release of BDNF and development of callosal axons

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Weeks 17 through 72 and beyond: Continued steady loss that gradually slows