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The three-dimensional crystal structure of GPx4 (PDB ID: 20BI) was used as the biological target for the docking analysis in this study

Treatment clues: Small clinical trials of N-acetylcysteine (NAC) , a glutathione precursor, show improvements in irritability and repetitive behaviors

Additionally, the reactive compounds, superoxide and peroxynitrite (ONOO ), can both inhibit GPx-1 (16, 41, 280)

Universidade de Vigo, Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Sciences, 32004, Ourense, Spain Corresponding authors: [email protected]

Proteins that undergo S-nitrosylation directly interact with NOS

After removal of the supernatant, the sample was diluted to 600 L with RIPA buffer