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[4] He was godfather to the eldest son of fellow actor Tom Chatto

Of the 4 microbes, 1 had not been reported at the time of isolation, but showed 99% 16S-rRNA gene sequence identity (Supplemental Figure 4B) with an organism recently isolated by others and classified as Emergencia timonensis (37)

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Both Genes were cloned via NcoI and NotI restriction sites into plasmid pETM20 and/or pETM11 (Dummler et al., 2005) yielding constructs pETM20- msadh or pETM11- msadh and pETM11- mdh
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