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This stoichiometric conversion serves as a quantifiable biomarker for autophagosome biogenesis monitoring, establishing LC3 lipidation as the gold-standard metric in autophagy assessment

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For reasons not known, and not explored, reciprocal crosses did not generate clones

The mpk-1 FBEa and FBEb interacted with both FBF-1 and FBF-2 in three-hybrid assays (Figure 3C and 3D) and bound to purified recombinant FBF-2 in gel shift assays (Figure 3E)