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Traverso et al., 2013)

[DOI] [PubMed] [Google Scholar] 61.Correia A.S., Cardoso A., Vale N

Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

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Its a non-invasive and safe procedure, and can be administered as a shot (in the muscle) or through the vein (by IV or push)