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First, various concentrations of cortisol were applied to TRT-HU1 Bmal1 -Luc cells to determine the dose-dependent effect of GC on the Bmal1 expression rhythm

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Through intact skin, permeation was near zero (Li et al., 2015)

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For comparison of the CMap data to our in vitro studies of the effects of GHK in primary lung fibroblasts, raw data for GHK-treated and control samples were downloaded from the CMap website and normalized using MAS5.0 with the Affymetrix CDF