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To this end, various structureactivity relationship (SAR) methodologies are employed, including, but not limited to, site-directed mutagenesis, rational computational design, high-throughput screening of synthetic peptide libraries, template-guided structural refinement, and mechanism-driven design strategies inspired by natural biological processes

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Autacoids such as histamine, prostaglandins and leukotrienes act via G protein-coupled receptors to induce activation of the G protein RhoA and phospholipase C, while exerting a depolarizing effect on smooth muscle plasma membrane that promotes calcium influx via voltage-gated calcium channels

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