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For the chromatographic metabolite separation, the Vanquish UPLC systems were coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA)

Additionally, ROS acts as secondary messengers in intracellular signaling cascades, modulating cellular processes such as antioxidant gene regulation, cell proliferation and survival, and autophagy [249,250,251]

Steinberg GR, Hardie DG

Mammary stem cells in domestic animals: the role of ROS

On one hand, HIF-1 upregulates the transcription and expression of the NLRP3 inflammasome, activates caspase-1, and induces gasdermin D (GSDMD)-mediated pyroptosis, leading to the release of pro-inflammatory cytokines such as IL-1 and IL-18 (Zhao P

The findings of Cochrans Q test ( p of Q = 0.983) (Figure 3) as well as the sensitivity analysis of leave-one-out (Figure 4) were all consistent