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doi: 10.3389/fcell.2022.1079548

Following washing with PBS, samples were analyzed using a fluorescent microplate reader (Ex: 540/15 nm, Em: 590/35 nm) with standardization of cell number by the Cell count normalization kit (C544, DOJINDO, Japan)

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The bait is typically expressed and purified from a recombinant system, such as bacteria or mammalian cells, to ensure a high yield of functional protein

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A better understanding of how such diverse influences trigger and interact with downstream pathological changes is essential for uncovering the full spectrum of PD pathogenesis