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Moreover, Sa--gal staining revealed that tissue and organ aging was attenuated, tissue damage was significantly reduced, and levels of tissue and organ fibrosisan aging-related phenotypewere decreased

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Animal studies have demonstrated that FOXO4-DRI can reverse many signs of aging, including: Improved muscle function and endurance Reduced kidney fibrosis and improved renal function Enhanced fur density and skin elasticity in aging mice Increased lifespan and overall resilience Additionally, FOXO4-DRI has shown promise in preventing neurodegeneration

Total RNA was extracted from the cell lysates using an EZ1 RNA Cell Mini Kit (Qiagen GmbH) on EZ1 Advanced XL automated RNA purification instrument (Qiagen GmbH) following the manufacturer's instruction

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