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To provide a biologically relevant source of copper, medium is supplemented with human ceruloplasmin at doses replicating low and high normal adult serum concentrations (250 and 500 mg/mL)

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doi:10.1038/s41366-022-01197-x

It is related to the binding of selenoprotein-P to the endothelium in sepsis, to the protein extravasation at the early phase of sepsis, and to downregulation of selenoprotein-P liver synthesis [33]