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Anemia pathophysiology in SCD begins with hemoglobin S (HbS) polymerization and sickling of red blood cells (RBCs), leading to intravascular and extravascular hemolysis, reducing RBC life span and the oxygen delivery capacity of blood, and resulting in tissue hypoxia and chronic ischemic organ damage

The 5 mL HEK293T cell suspension was combined with the mixture of 10 L of Nano-Glo Live Cell Substrate and 90 L of Nano-Glo LCS dilution buffer (Promega, Cat.# N2012)

AMPK-GLUT4 axis: mechanisms of anthocyanins in glucose metabolism Furthermore, the anthocyanin glucose-lowering effect is also linked to the activity of AMP-activated protein kinase (AMPK) and a rise in the GLUT4 transporter [91, 92]

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High-performance liquid chromatography (HPLC) and gas chromatography (GC) coupled with various detection techniques, such as ultraviolet-visible (UV-Vis) spectroscopy, fluorescence detection, or mass spectrometry (MS), enable the sensitive and selective analysis of thiols in diverse sample matrices