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However, like any medical or cosmetic procedure, glutathione injections may cause side effects, some of which are minor and manageable, and others of which are uncommon but serious

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Bacteriostatic water containing 0.9% benzyl alcohol, lab-grade, sealed, and within its expiry date Sterile syringes , 3 mL or 5 mL, depending on your volume, with 21-gauge needles Sterile nasal spray bottle calibrated to deliver a fixed volume per actuation (typically 0.1 mL, confirm before starting) Alcohol prep pads , isopropyl at 70% concentration Nitrile gloves Clean, flat work surface that can be fully disinfected before use Critical: Confirm the actuation volume before you do any calculations

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Finally, protein concentrations were determined using a Pierce bicinchoninic acid protein assay kit (Thermo Scientific, Waltham, MA, United States) (Komatsu et al., 2009