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To improve the recognition of AT domain of DEBS module 6 towards propargylmalonyl-CoA, Williams and coworkers generated saturation mutagenesis libraries of Tyr189 and Ser191 that are part of the specificity-determining Y A S H loop

Wu H, Liu J, Chen Z, et al

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Specific binding can be exhibited, e.g., by a low affinity targeting agent having a Kd of at least about 10 "4 M