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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s
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Nicotine has been shown to decrease TEER and disturb the BBB transport system, which leads to increased xenobiotic uptake (Hutamekalin et al., 2008
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