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First, as a retrospective single-center study, this study contended with the missing follow-up and unavailable data of SOD and GR

Each liver was homogenized in 3ml of 0.25 M sucrose per g of liver, and centrifuged at 3,000 and 10,000 rpm for 10 minutes respectively to precipitate cell debris

Annu Rev Pharmacol Toxicol 45, 51-88 (2005)

This effect was not dependent on a sustained ASC-2P presence but was abolished if the ASC2-P was added after the virus infection, indicating an immunomodulatory effect, rather than directly antiviral

Abstract Background Vitamin B 12 deficiency is common, and the incidence increases with age