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Cells were fixed with 4% PFA for 20 min at room temperature and then washed three times with 1X PBS (Gibco)

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Heart: Anatomy (MV) leaflets into the atrium by 2 mm above the plane of the MV annulus during ventricular systole Ventricular systole Cardiac Cycle: Most commonly characterized by myxomatous degeneration (Barlow syndrome): accumulation of glycosaminoglycans (GAGs) and alterations in collagen Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms

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