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Epidermal thickness of each sample was measured at 10 or 20 sites along 1-cm long, H&E- or anti-Ly6Gstained sections without histological artifacts, perpendicular to the long axis of the sections, encompassing the basal layer through all cell layers, excluding the stratum corneum, and averaged to obtain the mean thickness for each section

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Antigens were mixed 1:1 with squalene o/w emulsion (SE) (i.e., 250 L of antigen in PBS combined with 250 L SE) (Polymun, Klosterneuburg, Austria) and administered by two intramuscular immunizations in each quadriceps (2 250 L)

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doi: 10.1111/j.1600-065x.1998.tb01568.x 40 HorensteinALChillemiAZaccarelloGBruzzoneSQuaronaVZitoAet al

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