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Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer

After transfer of proteins onto nitrocellulose, membranes were Western blotted with the primary antibodies specified below
Oncogene 23 , 49754983 (2004)
Steatosis may be caused by the interaction of diet, genetic factors, gut microbiota, and lipogenesis through upregulation of lipogenic transcription factors, such as SREBP1c, etc

Patel, S.D