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For those requiring supplemental oxygen, particularly prolonged mechanical ventilation, it is critical that ventilatory gases are warmed and humidified to reduce these secondary consequences, along with optimising patient comfort

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On the AX12, AX iAECs were maintained in AX Alveolar Epithelial Barrier Medium (AlveoliX, Switzerland) supplemented with 1% penicillinstreptomycin (Thermo Fisher Scientific, Switzerland) for mono-culture studies and AX iAECs/hLMVECs were maintained in AX E2 Alveolar Barrier Medium (provided by AlveoliX) for all dual-culture studies

As per our previous protocol ( n = 10)

Cigarette Smoke-Induced Emphysema and Pulmonary Hypertension Can Be Prevented by Phosphodiesterase 4 and 5 Inhibition in Mice