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Following overnight incubation with the primary antibodies, membranes were washed five times for 15 min in TBST with mild shaking

Indeed, a large study done in the National Cancer Institute of the USA (60 cell lines and more than 100 anticancer drugs were examined) revealed a positive correlation between p53 status and cell sensitivity to cytotoxic drugs [57]

Emergence of small interfering RNA-based gene drugs for various diseases

The reporter ion intensities were defined as intensities multiplied by injection time (to obtain the total signal) for each isobaric labeling channel summed over all MS/MS spectra matching to the protein group as previously validated (Tyanova et al., 2016)

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The oxidative stress defensive factors such as FOXO1, are known to be inhibited by miR-27a, which is recognized as an oncogenic miRNA in gastric cancer (90)