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Yamada, 2000)
Sixty-nine high abundance and immunoglobulin proteins or immunoglobulin fractions that were not completely removed by the high-abundance protein depletion column were also detected
10.1186/s12967-023-04249-6 146 SinghP.WaghambareP.KhanT
10.1002/art.34677 210 ZhangJ.ShuaiX.LiJ.XiangN.GongT.ZhangZ
Immediately after cells were washed twice with 1 mM MgCl 2 in PBS (pH 6.0) and incubated with a staining solution composed of 1 mg ml 1 5-Bromo-4-chloro-3-indolyl--D-galactopyranoside (prepared in N , N -dimethylformamide), 0.12 mM K 3 Fe(CN) 6 , 0.12 mM K 4 Fe(CN) 6 and 1 mM MgCl 2 /PBS (pH 6.0) at 37 C overnight in CO 2 -free incubator
Setiap batch menjalani pengujian ketat untuk memverifikasi kandungan GSH dan memastikan karakteristik stabilitasnya.
