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FIGURE 2 Time also occurs in a linear setting, very well represented by the irreversible process of aging

10.1016/j.freeradbiomed.2006.02.013 70 LabrouN

Here, we employed murine pluripotent embryonic stem (ES) cells as a model system to dissect responses to AA stress

The proteins were electrophoretically transferred onto polyvinilidenedifluoride (PVDF) membranes (Bio-Rad, Hercules, California, USA) and probed with: anti-LC3 antibody (MBL), anti-P62 antibody (Sigma-Aldrich) or rabbit monoclonal anti-caspase 3 antibody (Invitrogen, Hillsboro, Oregon)

The comparison between qualitative data was made with two-sided Fishers exact test

4H) and was not incorporated into the necrosome complex (Fig