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Blots were blocked in Tris buffered saline containing Tween 20 (0.1% v/v) and 5% non-fat dry milk for 1 h at room temperature
Wu JY, Li YJ, Yang L, Hu YY, Hu XB, Tang TT, Wang JM, Liu XY, Xiang DX

Tretoquinol (trimetoquinol) was added as an example of a prohibited beta-2 agonist

An informed consultation with a doctor can help determine whether such treatments may be a suitable next step, or whether maintaining a conservative approach is more appropriate
