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Total protein extracts were obtained by homogenization of tissues using protein sample buffer [100 mM Tris-HCl (pH 6.8), 200 mM dithiothreitol, 4% sodium dodecyl sulfate (SDS), 0.2% bromphenol blue and 20% glycerol] and a classic protease inhibitor cocktail (Beyotime Institute of Biotechnology)

Mononuclear cells are easily isolated from EDTA/Li-heparin blood samples, and the CoQ10 status of these cells has been reported to correlate with that of skeletal muscle [70]

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Its high viscosity gel technology allows for even distribution over the eye surface, protecting it from external irritants and providing deep hydration without blurred vision

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