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Description
The additional 2-2.5 kg of loss comes predominantly from fat tissue, reflecting the lipolytic and anti-lipogenic mechanism
Supports physiologic IGF-1 production downstream of GH signaling

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Cells were washed three times with 1 ml PBS and lysed with 200 l 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 C for 5 min, followed by the addition of 800 l PBS
