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Th17 cells, a subset of T helper cells, contribute significantly to host defense by secreting pro-inflammatory cytokines (e.g., IL-17A/F, IL-21, and IL-22) (Sakaguchi et al., 2016)

Fan J, Kamphorst JJ, Mathew R, Chung MK, White E, Shlomi T, et al

(c) transferring the organic phase directly into the aqueous component, while homogenizing the combination with a high performance mixing apparatus, in particular a high-shear mixer

For instance, an instantaneous ligand addition could lead to sustained downstream responses due to the non-linearity of the downstream processes