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To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag.Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli

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Acetyl L-carnitine is an amino acid produced by your body and is essential for transporting fat into cells to produce energy

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Therefore 4-5 sprays are necessary