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littoralis can adjust to ITC-containing diets containing lower natural concentrations of these plant-derived toxins and not suffer declines in amino acid content due to ITC metabolism

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Mass spectrometry analysis Gel electrophoresis and in-gel digestion were carried out according to the standard procedures

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SnCE1 is N-terminally proteolytically processed in eukaryotic cells We expressed full length wild type SnCE1 and the non-acetylated mutants SnCE1 Y212A and SnCE1 C256A with C-terminal myc-His 6 -tag in HEK-293 cells and performed immunoblotting to analyze whether processing occurs from the N- or C-terminus (Fig

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