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Purification and Characterization The crude peptide was purified by preparative reversed-phase HPLC (RP-HPLC) using a gradient comprising buffer A (0.1% aqueous TFA) and buffer B (0.1% TFA, 90% acetonitrile, and water) on either a PerSeptive Biosystems VISION Workstation or a Gilson system

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Limitations include a predominantly female and White population

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