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Ferritin heavy chain-mediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition

17 , 1113e88 (2010)

Carrageenan-induced paw edema in rat elicits a predominant prostaglandin E 2 (PGE 2 ) response in the central nervous system associated with the induction of microsomal PGE 2 synthase-1

doi: 10.1073/pnas.0804336105

Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences

Linear detection range 0.01-3 M GSH equivalents with a detection limit of 10 nM GSH equivalents